This paper presents a RP-HPLC method for the simultaneous estimation of Cefixime and Levofloxacin in combined tablet dosage form. The process was carried out on C18 column (Luna 5 μm, 250 × 4.60 mm, i.d) using phosphate buffer pH 5.0 and Acetonitrile in the ratio of 70:30 v/v respectively as a mobile phase at a flow rate of 1.0 mL/min. Detection wavelength was fixed at isobestic point 283 nm. Linearity was observed in the concentration range of 20-100 μg/ml for Cefixime (r2 =0.9977) and 25-125 μg/ml for Levofloxacin (r2=0.9968). The retention time of Cefixime and Levofloxacin was found to be 2.898 and 6.091 minutes, respectively. The developed method is simple, precise, specific, robust, rapid, reproducible, and sensitive and it can be used for estimation of Cefixime and Levofloxacin in combined tablet dosage form.
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